Abstract

REVIEW ON REPORTED ANALYTICAL METHOD DEVELOPMENT BY UPLC AND HPLC OF SELECTED ANTI-BIOTIC DRUGS

M. Sivaganesh*, M. K. M. Abdhul Lathiff, G. Krishnamoorthy and R. Senthamarai

ABSTRACT

In this review article determines the different analytical methods for the establishment of selected Anti-biotic drugs such as (Metronidazole, Levofloxacin Hemihydrate, Ampicillin, Moxifloxacin hydrochloride and Clarithromycin) by using HPLC and UPLC. UPLC is a modern technique which gives a new direction for liquid chromatography. UPLC refers to ultra performance liquid chromatography, which covers mainly in three areas: “speed, sensitivity and resolution. Ultra performance liquid chromatography (UPLC) is applicable for particle less than 2μm in diameter for better resolution, sensitivity and speed compared with high-performance liquid chromatography (HPLC). Now a days Pharmaceutical industries are focusing for finding out new ways to the economy and shorten time for the development of drugs. UPLC analysis at the mean time gives the better quality of products and analytical laboratories are not exception in this trend. The separation and quantification in UPLC is done under very high pressure (up to 100M Pa). As compare to HPLC, the time and solvent consumption are less in UPLC. High performance liquid chromatography (HPLC) is an important qualitative and quantitative technique, generally used for the estimation of biological and pharmaceutical samples. It is the most versatile, safest, dependable and fastest chromatographic technique for the quality control of drug components. This article is prepared with an aim to review the comparison of the reported analytical methods of HPLC and UPLC of the selected Anti biotic drugs such as Metronidazole, Levofloxacin hemihydrate, Ampicillin, Moxifloxacin hydrochloride and Clarithromycin.[11,12]

Keywords: Method Development, High Performance Liquid Chromatography (HPLC), (Metronidazole, Levofloxacin hemihydrate, Ampicillin, Moxifloxacin hydrochloride, Clarithromycin), Ultra Performance Liquid Chromatography; High Separation Efficiency; Cost Effective; High