Abstract

EFFECT OF BENZYL ADENINE ON MULTIPLICATION OF CATHARANTHUS ROSEUS L. IN VITRO.

Dr. Hashim K. Mohammed Al-oubaidi*Aseel Salih Mohammed Ameen

ABSTRACT

Shoots about 1.5-2 cm of Catharanthus roseus L. were contained axillary buds exited and sterilized with ethanol 95 % for 1 min, then washed with DDH2O for 5 min, and rinsed with sodium hypochlorite at concentration 2% supplemented with 2 drops of tween-20 for 10 min then washed with DDH2O for 5 min. three times. The sterilized shoots cultured on modified MS medium supplemented with different concentration of (BA) and incubation at control conditions. Results showed that adding 2 mg/l (BA) to the medium caused significantly increasing of parameters compared with other treatments after 4 weeks of culturing. The average of shoots number was recorded (4.75), leaves number (9.25), the fresh and dry weight were recorded (1103.75, 112.00) mg respectively

Keywords: Catharanthus roseus, BA, multiplication.