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Abstract

SYNTHESIS AND CHARACTERIZATION OF AG-AU NANOPARTICLES FROM SPINACIA OLERACEA L. AND ITS ANTI-BACTERIAL AND ANTI-INFLAMMATORY ACTIVITY

Infant Jerry C. and Sheela S.*

ABSTRACT

Medicinal plants are the important compounds that are used in the preparation of traditional herbal medicine amongst rural sellers even till date. The bioactive compounds of medicinal plants are used as therapeutic agents where no satisfactory cure is present in modern medicines. Based on above ideas the present study is based on the synthesis and characterization of nanoparticles from Spinacia oleracea and the comparison of anti-bacterial and ant-inflammatory study of nanoparticles with hot and cold-water extract. The UV spectroscopy analysis shows the peak at the range of 431 nm and 554 nm for silver and gold respectively. The SEM analysis shows the clustered crystalline nature of the nanoparticles with fine spherical particles. Most of the particles are self-assembled with close compact arrangement. The maximum antibacterial activity of 23mm zone of inhibition was shown by nanoparticles against Bacillus subtilis (20mg/mL). The highest denaturation of albumin was found at 40mg/mL for nanoparticle with 59.7% when compared with hot, cold-water extract and standard. The highest anti-proteinase activity was found to be highest for nanoparticle with 36.9% when compared with others. These results reveal that the plants basically possess numerous bioactive efficiencies naturally, which could be explored more in future as an alternative for modern medicine which has numerous side effects.

Keywords: Medicinal Plants, Spinacia oleracea, UV spectroscopy, Scanning electron microscopy, anti-bacterial activity, anti-inflammatory activity.


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