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Abstract

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND DEVELOPMENT TECHNIQUES; A SHORT REVIEW

Muskan Kumari*, Mr. Pankaj Chasta, Dr. Gaurav Kumar Sharma and Dr. Kaushal Kishore Chandrul

ABSTRACT

High-performance liquid chromatography (HPLC) is a specific form of column chromatography which is generally used in biochemistry and analysis to separate, identify, and quantify the various drugs, drug active compound and drug related substance. The main intend of this article is to review and analyse the HPLC and its method of development techniques. High-performance liquid chromatography is generally depending on various factors such as synthetic route, solubility, polarity, pH and pKa values and most importantly chemical structures of the molecules and functional group activity etc.

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