Abstract

A REVIEW, ON ADVANCED INSTRUMENTATION TECHNIQUE FOR SUPERCRITICAL FLUID CHROMATOGRAPHY

Ajay D. Dongarwar*, Madhuri S. Nandgave, Anmol P. Talmale, Jaishree M. Bante, Ankit T. Nagwanshi Divyashri S. Shende, Divya P. Made and Dnyaneshwari M. Patle

ABSTRACT

In early days of supercritical fluid chromatography (SFC) it was categorized as high. Pressure or dense gas chromatography (HPGC or DGC). SFC Was first proposed in 1958 by J. Lovelock and first used in 1962 by Klesper. In this research paper we explain the effect of temperature, pressure and modifier Concentration on retention and selectivity, as well as how to make SFC more rapid and environmentally sustainable alternative to traditional high performance liquid chromatography methods utilizing organic solvents. These were explained by two example Separation of basic drugs paced These were explained by 2 examples, Separation of basic drugs by packed column SFC 2) Determination of caretinoids in microalgae using SFC.

Keywords: Pressure or dense gas chromatography (HPGC or DGC).