Abstract

PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL EFFECTS OF LEAF FRACTION OF RAUWOLFIA SERPENTINA PLANT

Prateek Porwal*, Sonali Paliwal, Harsh Sharma, Akash Sharma and Shalini Singh

ABSTRACT

Objectives: Rauwolfia serpentina has been used as a drug. Rauwolfia serpentina is a member of the Apocynaceae family. R. serpentina drugs are used to treat numerous neurological conditions, including vertigo, sleeplessness, schizophrenia, and sexual attack. The medicine is hypnotic, sedative, antihypertensive, and antimicrobial. The leaf extract can be utilized for pain-alleviation, liver pain, diarrhea, and the expulsion of intestinal worms. Material and Methods: Leaf of R. serpentina collects from the street of Lucknow UP, India. Methods: The findings of phytochemical screening of Rauwolfia serpentina methanol Leaf extracts are provided. Qualitative analyses were conducted for alkaloids, carbohydrates, flavonoids, steroids, phenols, and other phytochemicals to know the existence in those crude plant extracts of primary and secondary metabolites. The natural extracts and their corresponding fractions, the preliminary antimicrobial test was conducted by the agar well diffusion test described in the plant part's antibacterial, antibacterial activity (i.e., raw extracts and fractions). Results: Qualitative testing of R. serpentina methanol extracts revealed alkaloids, saponins, tannins, steroids, and phenols. Qualitative analysis of R. serpentina and leaf extract suggesting that tannins, saponins, flavonoids, alkaloids, and starch were found in leaf extract gave a positive test to reduce sugar, tannins, alkaloids, and starch. The plant part's antibacterial antibacterial activity (i.e., raw extracts and fractions) was tested against the above standard clinical isolates. Conclusions: The phytochemical study of R. Serpentina leave extracts shows alkaloids, saponins, tannins, steroids, flavonoids, and phenols. The quantitative evaluation of Rauwolfia serpentina phytochemical elements suggests that the plant sample contains 2% fat, 12.4% alkaloid, and 7.35% saponin. GC-MS n-hexane extract chromatograph of Plant components' antibacterial and antifungal effects (against M. canis) enable the use of plants in ethnomedicine to treat ailments like gastroenteritis, GIT, and skin infections. Further investigations are therefore needed to determine the specific chemicals responsible for these effects.

Keywords: R. Serpentina, Antimicrobial, Herbal plant, Clinical isolation.