ROLE OF PROTEIN OXIDATION AND LIPID PEROXIDATION MARKERS STATUS IN NIDDM PATIENTS
Kalaivanam. K N, Santhosh Kumar.N*, Bheemasen. R ,
Balu Mahendran.K
ABSTRACT
AIM & Objective: The link between hyperglycemia, enhanced free
radical activity, and the complications of diabetes is unknown. The
purpose of this study is to evaluate the levels of Protein Carbonyl,
malondialdehyde (MDA) measured as thio-barbituric acid-reactive
substances (TBARS), an index of protein oxidation, lipid peroxidation
& microalbumin in patients of type 2 diabetes without complications
compare with normal subjects of the same population. Methodology:
We recruited 60 type 2 diabetic subjects without complications and
with poor metabolic control and 60 age-matched controls with good
metabolic control. Levels of Protein Carbonyl, glucose, total
cholesterol, HbA1C, and MDA as TBARS, micro-albumins were
determined. Results: Diabetic patients had higher levels of blood glucose (P<0.001), HbA1C
( P<0.001), Protein Carbonyl( P<0.001), microalbumin ( P<0.05)and MDA (P<0.001) than
control subjects. The total cholesterol of the control subjects and diabetic patients did not
differ. There was no correlation between the family history in diabetics and elevation in
either HbA1C or MDA levels. Conclusion: To clarify the levels of protein oxidation markers
such as protein carbonyl (PCO), increased levels of Protein Carbonyl, MDA may be a useful
marker of oxidative stress. The enhanced lipid peroxidation & protein oxidation leads to an
increase in free-radical activity in type 2 diabetics. This increase in free-radical activity in
type 2 diabetes mellitus along with insulin resistance can lead to activation of stress-sensitive
pathways, which may play an important role in the complications of diabetes.
Keywords: Protein Carbonyl (PCO), malondialdehyde (MDA), type 2 diabetes, thiobarbituric acid-reactive substances (TBARS), HbA1C.
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