Abstract

COMPARATIVE EVALUATION OF ANTHELMINTIC ACTIVITY OF NERIUM INDICUM, MILL FLOWER EXTRACT AND PUNICA GRANATUM, LINN PEEL AND SEED EXTRACT IN 1:1 RATIO AND THEIR PHYTOCHEMICAL SCREENING.

Mr. Sachin Annasaheb Nitave*, Mrs. Vishin Ashish Patil

ABSTRACT

Nerium olender belongs to family Apocyanaceae and Punica Granatum belongs to family Lythraceae. The objective of the present work was to identify the phytochemical constituents and to compare the in vitro anthelmintic potency of the aqueous and ethanolic extract of Nerium olender flower and Punica Granatum peel and seed in 1:1 ratio using Indian earthworms (Pheretima posthumad). The various concentrations ( 25, 50, 75 & 100mg/ml) of the aqueous and ethanolic extract were tested in vitro for anthelmintic potency by determination of time of paralysis and time of death of worm. Albendazole (25mg/ml) used as standard. The result of present study indicates ethanolic extract of Nerium olender and Punica Granatum paralyze earthworm and also caused its death after some time. Thus, the present study indicates that Nerium olender and Punica Granatum are anthelmintic and Nerium olender has more anthelmintic activity with compare to Punica Granatum. The phytochemical investigation of aqueous and ethanolic extracts of flower of Nerium olender showed the presence of active chemical constituents such as Carbohydrates, Alkaloids, Flavonoids, Steroids, Cardiac Glycosides, Anthraquinone Glycosides, Proteins, Amino Acids, Triterpenoids and Tannins while that of Punica granatum peel and seed show presence of alkaloids, tannins, flavanoids, steroids, , cardiac glycosides and terpenoids.

Keywords: Death, Phytochemicals, Nerium olender, Punica Granatum, Paralysis, Pheretima posthumad.