Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS: JULY ISSUE PUBLISHED
  • JULY 2021 Issue has been successfully launched on 1 July 2021.

Abstract

OPTIMIZATION STUDIES ON ALKALINE PROTEASE OF ASPERGILLUS NIGER ISOLATED FROM SEASHORE SOIL OF BAY OF BENGAL

Yepuru Suman Kumar, Sujatha Gundeboina, Lingamgunta Lakshman Kumar and Kedam T. Raju*

ABSTRACT

Due to the importance alkaline proteases in biotechnological applications, a number of microorganisms explored. Therefore, this study, we isolated Aspergillus niger from soil biome and various parameter such incubation, temperature, pH, substrate and some metal ions, chelators and inhibitors on enzyme activity were determined. The study reveals that the strain was identified by morphological and screened on skim milk agar plates at 37o C for 72 h and confirmed through protease assay. The optimum yield of this enzyme was obtained from the appropriate fermentation conditions like pH ranging from 6.0-8.5, temperature at 20-40oC and incubation for 24, 48, and 72 h respectively was studied. In addition, the maximum enzyme production obtained from solid state fermentation under 10% wheat bran substrate compared to other agro-waste solid substrates such as sesame oil cake (SOC) and cotton seed oil cake (COC). Furthermore, protease was significantly activated by metal ions such as Fe 2+, Zn2+, Ca 2+ while it was inhibited by the metal ion chelator phenylmethylsulphonyl fluoride (PMSF), ethylenediaminetetraacetic (EDTA) and SDS. Our findings suggest the potential of this isolate for protease production and that this enzyme may be suitable for biotechnological purpose.

Keywords: Alkaline proteases; Aspergillus niger; Sesame oil cake; Cotton seed oil cake; Biotechnology.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More