Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS: JULY ISSUE PUBLISHED
  • JULY 2021 Issue has been successfully launched on 1 July 2021.

Abstract

RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF DAPAGLIFLOZIN PROPANEDIOL MONOHYDRATE AND SITAGLIPTIN FROM SYNTHETIC MIXTURE

Thakkar Jay*, Neha Tiwari and Pragnesh Patani

ABSTRACT

A simple, accurate and precise HPLC method was developed and validated for Estimation of DAPAGLIFLOZIN PROPANEDIOL MONOHYDRATE (DPM) and SITAGLIPTIN (SIT) from synthetic mixture. The separation was achieved under optimized chromatographic condition on Hypersil ODS C18 (250*4.6 mm, 5 μm) column with mobile phase consisting of acetonitrile: Buffer (Potassium dihydrogen phosphate 0.02M and pH was adjusted by ortho phosphoric acid) (pH 4) (70:30v/v) with gradient elution at a flow rate of 1 mL/min using column ambient temperature with UV detection at 254nm. The retention time for Dapagliflozin propanediol monohydrate and Sitagliptin were about 2.4 and 6.9 minutes respectively. The linearity was found to be in the concentration range of 5-25 μg/mL for Dapagliflozin propanediol monohydrate and 50-250 μg/mL for Sitagliptin . The % recoveries at 50%, 100% and 150% were found to be within the limit of 98-100%. The method was validated as per ICH guidelines; and the values were found to be within the limits. So, the proposed method was found to be simple, linear, accurate, precise, robust and specific.

Keywords: Dapagliflozin propanediol monohydrate (DPM), Sitagliptin (SIT), Validation, Synthetic mixture, HPLC.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More