Abstract

INVESTIGATION ON TISSUE REPAIR EFFECTS AND ANTI-MICROBIAL ACTIVITY OF LARVAL BLOOD EXTRACT FROM Myrmeleon formicarius

Devasahayam Joyce Hellen Sathya*, Kollengattuparambil Mohandoss Praveen, Gopalakrishnan Navaraj, Amirtham Jacob Appadorai Ranjit Singh and Antony Joseph Jeyadoss

ABSTRACT

The present study is focused on investigating the tissue repair effects and the antimicrobial properties of the Insect Blood Extract collected from the larvae of Myrmeleon formicarius (antlion). The study is also focused on determining cytotoxic effects of antlion extract. The blood extract was collected by puncturing with tweezers and its properties were analyzed by GC-MS and SDS-PAGE. The Cytotoxicity and Wound healing property of the Insect Blood Extract were studied by MTT and in-vitro wound healing scratch assay on VERO cell line and 3T3 cell line respectively. Antibacterial activity of Insect Blood Extract was analyzed by well diffusion method and also by hypothetical statistical analysis. The results revealed that the Insect Blood Extract has good Tissue regenerative property. SDS-PAGE confirmed the presence of Chaperone protein. The IC50 value of extract was found to be 60.28 μg/ml. MIC (minimum inhibitory concentration) was found to be 40μl/mL,80μl/mL,60μl/mL on P.aeruginosa, S.aureus, E.coli respectively. Maximum inhibition effect was observed at the concentration of 80μg of insect blood extract for all the bacterial strains. F ratio value was determined to be 0.0653 using ANOVA (Analysis of variance) test. The presence of Chaperone protein present in insect blood extract was found to be responsible for the wound healing property. From t-Test on comparing the mean zone diameter of three bacterial strains it was evident that insect extract showed high inhibition on S.aureus than on E.coli and P.aeruginosa. Anova table results showed that Fobserved

Keywords: Insect Blood Extract, Cytotoxicity, Chaperone, Apoptosis, Tissue regenerative property, Wound healing property, statisticalanalysis.