Abstract

IN VITRO ANTIOXIDANT ACTIVITY OF CALAMUS ROTANG LEAVES: EFFECT OF EXTRACTION SOLVENTS

Tanaya Ghosh and Prasanta Kumar Mitra*

ABSTRACT

In vitro antioxidant activity of Calamus rotang (C. rotang) is known in literature. But comparative study on effect of extraction solvents on in vitro antioxidant activity of C. rotang leaves is not reported in available literature. Therefore, aim of present work was to see the effect of different extraction solvents on in vitro antioxidant activity of C. rotang leaves. C. rotang leaves were collected from the medicinal plant garden of the North Bengal University and identified by the taxonomist. Solvent extraction of the leaves was done separately by using chloroform, petroleum ether, methanol, ethanol, ethyl acetate and acetone. Extracts were separately dried and processed for in vitro antioxidant activity through superoxide anion generation by xanthine-/xanthine oxidase assay, linoleic acid peroxidation assay and by DPPH photometric assay. Quercetin was used as standard antioxidant. Results showed that methanol extract of C. rotang leaves had maximum antioxidant activity against the methods used which was comparable to that of quercetin.

Keywords: Calamus rotang Leaves, solvent extractions, xanthine-/xanthine oxidase assay, linoleic acid peroxidation assay, DPPH photometric assay.