Abstract

PHYTOCHEMICAL SCREENING AND THIN LAYER CHROMATOGRAPHIC PROFILE OF LEUCAS BIFLORA LEAVES USING ETHANOL SOLVENT

Twinkle S. Zade*, Kamala K. Chandak and Milind J. Umekar

ABSTRACT

The current studies purpose is to conduct of phytochemical screening and thin layer chromatography of ethanolic extract of leucas biflora leaves. The plant has been reported to be used traditionally for the decoction of mature leaves is used as an eye drop twice a day in treatment of Conjunctivitis. This juice is effectively used in treatment of Nasal bleeding and beneficial for women suffering from white discharge. Leaves is use to relief of headache and the paste of leaves is effective for skin diseases and wound healing. The planting material was collected, the leaves were dried in the shade and ground into a coarse powder. It was then extracted with ethanol solvent by soxhlation. The extract was subjected to conduct phytochemical Screening to detect the presence of carbohydrates, lipid, glycosides, terpenoids/steroids, tannins, phenolic compound and flavonoids A number of different solvent system were tried for developing TLC profile of ethanolic extract of L. biflora leaves and the better separation of components was observed in Ethyl acetate: Petroleum ether: Benzene: Formic acid (10: 6: 3: 1) solvent system. 5 components where found to be separated with corresponding Rf values 0.111, 0.5, 0.592, 0.795, 0.962. In the future, these phyto-constituents can be separated by column chromatography and can be identified by mass spectroscopy, infrared spectroscopy, nuclear magnetic resonance spectroscopy etc. Their pharmacological activity can be tested and can be classified into appropriate dosage form

Keywords: Leucas biflora, Ethanol, phytochemical screening, TLC.