Abstract

CALLUS INDUCTION AND PLANT REGENERATION OF MEDICINAL PLANT CATHARANTHUS ROSEUS L. IN VITRO

Alaa Jabbar Taha*

ABSTRACT

The present study indicate the possibility of the development of callus when culture leaf and stem of Catharanthus roseus (L.) on MS media supplement with different concentrations of NAA+BA. Sterilized explant were inoculate and incubated in culture under different condition (light and dark). MS media with a concentration of BA1mg/l and NAA1.5mg/l was superior in lighting conditions when cultured leaves, giving the highest percentage of callus induction 91% and using this combination of induced callus as maintenance media, the highest fresh and dry weight significantly reached to 610.7 and 169.32 mg respectively. Induced callus cultured in MS media supplemented with 2mg/l kin and 1mg/l IAA, had a significant effect on the formation of the vegetative branches, the percentage of vegetative branch formation in this combination reached to highest significantly 41%. Branches cultured in MS media equipped with 1.5mg/l IAA for rooting, the percentage of rooting reached to the highest rates and significantly to 69%, the number of roots/branches and root length 6.93 root and 4.19cm respectively.

Keywords: Explant, light and dark condition, callus induction, regeneration, rooting, PGRs.