Abstract

STUDY OF ANTIOXIDANT ACTIVITY OF EUPHORBIA HIRTA LINN WHOLE PLANT IN MICE.

Madhusha Reddy Y, Praveen Kumar Uppala*

ABSTRACT

The main objective is to investigate antioxidant activity of extract of Euphorbia hirta whole plant in mice both in-vitro and in-vivo methods. The in-vitro antioxidant activity was carried out to evaluate reducing power,Superoxide anion scavenging activity, hydroxyl radical scavenging activity, nitric oxide radical scavenging activity. The degree of protection was determined by measuring levels of biochemical markers like SGOT, SGPT, ALP, Bilirubin, the histopathological studies were also carried out1. Significant increase in GSH level and scavenging activity and decreased lipid peroxidation The results were comparable with the standard. In vitro antioxidant studies were carried out. Administration of alcoholic extract of Euphorbia hirta whole plant at 100μg dose has demonstrated dose dependent increase in reducing power, which is comparable to that of std. sodium metabisulphate at 25μg. Similar results are obtained in case of superoxide anion, hydroxyl radical scavenging activity,Nitric oxide radical scavenging activity. Alcoholic extract of Euphorbia hirta whole plant has significant invitro lipid peroxidation and scavenging activity among other polar extracts.

Keywords: Euphorbia hirta whole plant; marker enzymes; antioxidant ; superoxide anion; hydroxyl radical scavenging.