Abstract

ANTIBACTERIAL ACTIVITY (IN VITRO) OF RHIZOMES OF CURCUMA LONGA(DAY AND NIGHT COLLECTED) AND CURCUMA AROMATICA AGAINST ON PATHOGENIC BACTERIA

*Debbarma Promod and Jaiswal Mohanlal

ABSTRACT

The Aqueous, Ethanol and Petroleum ether extracts of the rhizomes of Curcuma Longa and Curcuma Aromatica (Family-Zingiberaceae) was screened for antimicrobial activity against five human pathogenic bacterial strains, gram positive bacteria i.e Streptococcus pyogenes, Staphylococcus aureus, Beta -haemolytic streptococcus and gram negative bacteria i.e Pseudomonas aeuriginosa, Klebshiella Pneumonia which are most of bacteria those are mainly responsible causing for the skin and respiratory tracts infections commonly found in local community. Aims-The aim of the present study was to evaluate the antimicrobial activity of various solvent extracts of Curcuma Longa and Curcuma aromatic. Methods- In vitro antimicrobial study of formulations was carried out by using the Agar well diffusion method for the assessment of anti- microbial activity. Sample prepared as each sample (10 mg/ml) concentration and Ciprofloxacin (5μg/ml) was used as standard antibacterial drug. Results and discussion- Activity index against S. pyogenes- ethanol extract of all three samples are shows equal significant sensitive, i.e. DH(0.66), NH(0.66) & VH(0.66 Pet.ether and Aq.extract of DH, NH & VH are not found sensitive. Activity index against P.aeuriginosa- ethanol extract of DH (0.75) & NH (0.75) both are equal sensitive and less than VH (0.83). Pet. Ether extract of VH (0.54) is only show sensitive. Aq. extract- NH(0.83) is show only sensitive. So overall found NH (0.83/Aq.ext) more than DH (0.75/Ethanol ext), and NH (0.83/Aq.) and VH (0.83/Ethanol ext.) both are equal sensitive. Activity index against S. aureus- ethanol extract of DH (0.6) is more than NH (0.55) and less than VH (0.66). Pet. Ether- DH (0.55) is less than VH (0.65). Aq.extract- not found sensitive. So, overall found VH (0.65/Pet. ether) more than DH (0.55/ Pet. ether) all are sensitive. Activity index against K. Pneumonia- ethanol extract DH (0.54) and NH (0.54) both are equal sensitive and less than VH (0.63). Pet. ether extract- DH (0.72) more than VH(0.59). NH is not found sensitive. Aq. extract- all three samples are shows insignificant sensitive. So, overall found DH (0.72/Pet. ether) is more than VH (0.63/Pet. ether). Activity index against Beta-haemolytic streptococcus- ethanol extract- DH (0.68) & NH (0.68) both are equal significant sensitive and less than VH (0.72). Pet.ether extract- DH & NH shows insignificant, only VH (0.63) show sensitive. Aq. extract- all three samples are shows insignificant. Conclusion-The test drugs of present study found effective in certain strains of bacteria and definitely need validation by it further more studies in animal models and in clinical studies in Respiratory tracts infections and skin infections etc, which are occurred by these bacteria.

Keywords: Antibacterial activity, Curcuma Longa, Curcuma aromatic, Aqueous, Methanol and Petroleum ether extracts.