Abstract

EVALUATION OF ANALGESIC EFFECTS OF LEAF EXTRACT OF MORINGA OLEIFERA IN SWISS ALBINO MICE

Dr. Tshema V.*, Dr. S. Anusha and Dr. R. Mani

ABSTRACT

Background: Pain is one of the common reasons to seek medical care. Even though NSAIDS and opioids are effective in pain management, they have many adverse effects. Herbal medication may provide an alternative therapy. Moringa oleifera (Drumstick plant) is known since antiquity and its medicinal properties are used traditionally in India. Aim: To evaluate the analgesic effects of Ethanolic leaf extract of Moringa oleifera (EMO) in Swiss Albino Mice. Materials and methods: This study was approved by Institutional Animal Ethical Committee (IAEC). 24 Swiss albino mice of either sex, 6 weeks old, 25 to 30 grams each were divided into 4 groups with 6 mice in each group. Group I (control) received normal saline 2ml/kg orally. Group II (standard) received morphine 1mg/Kg intra-peritoneally. Both Group III and Group IV were test groups, that received EMO Leaf Extract orally for 7 days, at the dosage of 200mg/Kg (0.1 ml) and 400mg/Kg (0.2ml) respectively. The analgesic effect was evaluated using Eddy’s Hot plate. Statistical analysis was done using ANOVA and Probability (P) value. Result: The reaction time of groups was observed using Eddy’s Hot Plate. Groups II (morphine), III (EMO-200 mg/kg) & IV (EMO-400 mg/kg) showed 10.4 seconds, 7.35sec & 8.45 sec respectively at 60 minutes. Among the test groups, Group IV (400 mg/kg of EMO) showed the maximum reaction time (significant with p-value <0.05). (standard) received morphine 1mg/Kg intra-peritoneally. Both Group III and Group IV were test groups, that received EMO Leaf Extract orally for 7 days, at the dosage of 200mg/Kg (0.1 ml) and 400mg/Kg (0.2ml) respectively. The analgesic effect was evaluated using Eddy’s Hot plate. Statistical analysis was done using ANOVA and Probability (P) value. Result: The reaction time of groups was observed using Eddy’s Hot Plate. Groups II (morphine), III (EMO-200 mg/kg) & IV (EMO-400 mg/kg) showed 10.4 seconds, 7.35sec & 8.45 sec respectively at 60 minutes. Among the test groups, Group IV (400 mg/kg of EMO) showed the maximum reaction time (significant with p-value <0.05).

Keywords: Analgesic, Drumstick, Eddy’s Hot Plate, Mice, Moringa oleifera leaf extract.