Abstract

PHYTOCHEMICAL ANALYSIS, ANTIBACTERIAL AND ANTIHYALURONIDASE ACTIVITY OF THREE INDIGENOUS MEDICINAL PLANTS

*Asha Gangadharan*; Elizabeth Jacob; Densely Jose

ABSTRACT

Medicinal plants in traditional systems of medicines produce a diverse range of bioactive molecules, making them a rich source of drugs. In this study phytochemical analysis of medicinally important plants like Aloe vera, Leucas aspira and Biophytum sensitivum and their antibacterial and antihyaluronidase effect were studied. The effect of alcoholic and aqueous extracts of extracts on different microorganisms were done by Kirby Bohr agar well diffusion assay and the inhibition on bovine testicular hyaluronidase was tested by Elson – Morgan assay. Phytochemical analysis of the alcoholic extracts of plant extracts revealed the presence of alkaloids, proteins, steroids & terpenoids, in addition to carbohydrate, flavonoids, phenolic compounds and oils in aqueous extract. The antibacterial assay showed that all the ethanolic extracts had profound anti bacterial activity at lowest minimum inhibitory concentration and the zone of inhibition increased with increase in concentration of extract. Leucas aspera, Biophytum and Aloevera had significant activity against Klebsiella, S.aureus and Bacillus. The MIC and MBC studies showed that the extracts were bacteriostatic at lower concentrations and bactericidal at higher concentrations. The percentage of inhibition of alcoholic and aqueous extracts on the activity of Bovine Testicular Hyaluronidase at concentrations 5, 10, 15 mg/ml was found to be 71.80%, 81.43%, 94.29% and 79.22%, 87.48% and 91.31% respectively for A. vera and 38.21%, 43.05%, 59.45% inhibition and 73.51%,79% and 96.43% inhibition respectively for B sensitivum and 56.54, 73.24, 88.33 and 11.67, 29.97 and 33.32% for Leucas aspira respectively. The study of potent antibacterial and anti hyaluronidase activity confirmed the potential of Leucas aspira, A.vera and B.sensitivum as anti-allergic, contraceptives, anticancer, anti-inflammatory and anti-arthritis agent.

Keywords: Phytochemical analysis, Anti Bacterial, Anti hyaluronidase activity, Biophytum sensitivum, Leucas aspira, Aloe vera