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Kasture Veena S, Mhaske Sharayu D*, Pathan Mallika A, Mantode Ashwini B


The geometrical correction method eliminates or reduces background irrelevant absorption that may be present in sample from biological origin. A three point geometrical correction method was used for quantification of Metaxalone from biological samples(egg albumin). The method was developed at three different wavelengths 260nm, 270.80nm, 282 nm for Metaxalone by using egg albumin as biological media. The proposed method showed linear relationship at three wavelengths in the range of 30-180 μg/ml with regression coefficient of 0.994, 0.995, 0.993. The % RSD for precision at three wavelengths was found to be 1.442%, 1.129% and 1.216%.% RSD for ruggedness and robustness at three wavelengths were found to be 1.418%,0.641%,1.552% and 1.558%, 0.696%, 1.554%. The accuracy at three wavelengths was found to be 93.46±1.27, 94.66±0.89 and 93.25±0.87. The proposed method was found to be accurate, precise, rugged and robust due to the lower values of %RSD.The proposed method was validated as per ICH guidelines. For biological media (egg albumin), corrected absorbance (D value) was calculated. D value showed linearity with concentration showing regression coefficient 0.983. A three point geometrical correction UV spectrophotometric method was developed and validated as per ICH guidelines using egg albumin as biological medium for quantification of Metaxalone.

Keywords: Metaxalone, Geometric correction method, Egg albumin.

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