Abstract

PTBP1 REGULATES THE ALTERNATIVE SPLICING PROCESS OF PPP3CC AND PROMOTES THE PROLIFERATION AND MIGRATION OF LIVER CANCER CELLS

Xiuli Zhang* and Lianghua Shen

ABSTRACT

Liver cancer is one of the main diseases that cause deaths in urban and rural residents in the world. Further clarifying the molecular mechanism of the occurrence and development of liver cancer has a very important guiding role in the treatment of liver cancer. Studies have shown that alternative splicing plays an important role in tumor metastasis. We preview found that the splicing protein PTBP1 is related to the development of liver cancer cells. In this paper, we screened and identified the downstream target genes of PTBP1 in liver cancer cells. The preliminary results indicated that PTBP1 may regulate the alternative splicing of PPP3CC and thus affect the development of liver cancer. We specifically blocked PPP3CC-Long and PPP3CC-Short isoforms respectively, subsequent CCK8 and migration experiments showed that specific knockout of PPP3CC-S isoforms could significantly inhibit the proliferation and migration of liver cancer cells. This study focusses on the process of PTBP1 regulating PPP3CC alternative splicing, and clarifying the functional differences between PPP3CC isoforms in the biological processes of liver cancer cells. In conclusion, our results suggest that PTBP1 regulates the alternative splicing process of PPP3CC and promotes the proliferation and migration of liver cancer cells, which will provide new ideas for elucidating the development mechanism of liver cancer cells.

Keywords: Liver cancer, Alternative splicing, PPP3CC, Proliferation, Migration.