Abstract

PATHOGENESIS AND RELATIONSHIP BETWEEN EARLY INDICATION ENZYMURIA FROM PROXIMAL RENAL TUBULE AND KIDNEY ENZYME ACTIVITIES IN SERONEGATIVE RHEUMATOID ARTRITIS

Dejan Spasovski*

ABSTRACT

Introduction: To determine the effects of non-treated seronegative rheumatoid arthritis (RA) on proximal renal tubule, sensitivity of Alanine aminopeptidase (AAP), -glutamyltransferase (--GT), β2 microglobulin in urine β2M), as well as relation with rheumatoid factor (RF) and C-Reactive protein (CRP), DAS 28 disease activity index. Methods: RF was determined by agglutination test (Latex RF test, while kinetic methods were used for determination of Alanine aminopeptidase (AAP) and -glutamyltransferase (-GT), as well as MEIA (Microparticle Enzyme Immunoassay) to determine β2 microglobulin in urine. Samples (serum and urine) of 70 participants were examined (35 RA not treated, 35 health control group). Results: In 35 RF negative RA, AAP enzymuria was present in 12 (34.28%) patients, -GT was present in 7 patients (20%), while β2 microglobulin was present in 3 patients (8.57%). In the healthy control group, 4 patients showed AAP positivity (11.42%), 2 patients -GT positivity (5.71%) and 1 patient showed presens of 2 microglobulin in urine (2.85). RF was not present in any patient (0%). Conclusion: AAP has a higher sensitivity of -GT and 2 microglobulin in the detection of asymptomatic renal lesions in non trated seronegative RA.

Keywords: Alanine aminopeptidase, (AAP); ?-glutamyl transferase (?--GT), ?2 microglobulin (?2M); Rheumatoid arthritis (RA), Rheumatoid factor (RF).