Abstract

AMELIORATIVE EFFECT OF CELASTRUS PANICULATUS AGAINST CCL4 INDUCED HEPATOTOXICITY

Arpita Choudhary*, Dr. Sailesh Narayan, Dr. Shailendra Bindaiya and Dr. Shailendra Lariya

ABSTRACT

The present study was performed to evaluate the ameliorative effect of celastrus paniculatus against CCl4 induced hepatotoxicity in rats. In the present study, Celastrus paniculatus was selected for hepatoprotective activity owing to its traditional use. The liver function is assessed by determining the activities of SGOT, SGPT, SALP, i.e. enzymes present in the cytoplasm. In case of hepatotoxicity, the enzymes move into the bloodstream and their amount confirms the liver damage extent. The plant material was extracted by continuous hot percolation Soxhlation and the percentage yield calculated by the following formula was found to be 2.41 % (by ethanol). Phytochemical screening of ehanolic extract of Celastrus paniculatus showed the presence of carbohydrate, alkaloid, protein, flavonoids, tannin and phenolic compound, saponin, triterpenoids and steroids and absence of glycoside. In case of acute oral toxicity study when animals were treated with 5, 50, 300, and 2000 mg/kg b.w. of dose for 72 hour there was no mortality and any behavioural changes, so the dose will be choosen as 200 mg/kg b.w. on the basis of LD50. Administration of ethanolic extract showed a substantial decrease in SGPT, SGOT, SALP level (p <0.05) at a dose of 200mg/kg. Similarly, treatment with hepatoprotective drug such as silymarin showed a significant decrease in SGPT, SGOT and SALP levels. Therefore, it was reported that Celastrus paniculatus extract treatment normalize few morphological features of the liver in rats with CCl4- induced hepatotoxicity.

Keywords: Ethanolic extract of Celastrus Paniculatus, Soxhlation, Hepatotoxicity, Silymarin, CCl4 induced hepatotoxicity.