Abstract

A- AMYLASE INHIBITION ASSAY OF AQUEOUS FOLIAR EXTRACT OF PROSOPIS JULIFLORA

Neha Chauhan*, Yash Dutt and Nikita Jaiswal

ABSTRACT

Prosopis juliflora is a xerophytic evergreen tree. This herb is well known for its medicinal value. The plant has been reported to treat oral problems like toothache. The leaves were used to treat Asthma, bronchitis conjunctivitis and skin diseases, blood and veneral diseases High concentration of glucose in blood causes diabetes. It is caused by deficiency in production of insulin by pancreas. High concentration of glucose in blood may damage vital organ of body. Screen off treating diabetes is to decrease the carbohydrate hydrolysing enzyme such as α-amylase and α-glucosidase. Both enzymes are necessary for digestion of carbohydrate. Amylase is a digestive enzyme which breaks bond between sugar molecule in polysaccharide through hydrolysis reaction and decreases the level of glucose in blood. The phytochemicals present in plants are responsible for preventing disease and promoting health have been studied extensively to establish their efficacy to understand the underlying mechanism of their action. Result of this study revealed that leaves extract showed α-amylase inhibition in a dose dependent manner. The extracts showed maximum inhibition at a concentration of 10mg/ml and which is decreased with decreasing concentrations i.e. 2.5 and 1 mg/ml. In conclusion, more research is required for developing a potential and valuable antidiabetic therapy by using α- amylase inhibition.

Keywords: Prosopis juliflora, ?-amylase, diabetes.