Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS: SEPTEMBER ISSUE PUBLISHED
  • SEPTEMBER 2021 Issue has been successfully launched on 1 September 2021.

Abstract

IMMOBILIZATION ON CHITIN AND POLYETHYLENE OF INVERTASE OBTAINED FROM YEAST GROWN IN MOLASSES BY FED-BATCH PROCESS

Michele Vitolo*

ABSTRACT

Saccharomyces cerevisiae is largely used in industry and in biochemical studies. The main product extracted from this yeast is invertase, which has many applications, for instance in syrup production in either soluble or immobilized form. This study dealt with invertase production from S. cerevisiae grown in blackstrap molasses by fed-batch fermentation. Fermentation parameters as specific rate of cell growth (), substrate consumption, generation time, and invertase activity of intact yeast cells were determined. The invertase present in cells obtained by exponential fed-batch culture was extracted and purified. The purified invertase was immobilized on chitin (adsorption plus cross-binding with glutaraldehyde) and polyethylene (covalent binding). The kinetic (KM and Vmax) and thermodynamic (Ea, G, H, and S) constants of all forms of invertase were calculated.

Keywords: Invertase, chitin, polyethylene, yeast, molasses, fed-batch.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More