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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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WJPPS: MAY ISSUE PUBLISHED

May Issue has been successfully launched on 1 May 2024.

WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

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WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

Abstract

ENZYMATIC GLYCOSYLATION OF CURCUMIN USING GLYCOSYLTRANSFERASE OLED VARIANT ASP FROM STREPTOMYCES ANTIBIOTICUS

Fo-Lan Lin, Xiao-Lu Wang, Si-Si Li, Qi-Qi Wang and Ren-Wang Jiang*

ABSTRACT

The objective of this study is to explore the glycosylation of curcumin with glycosyltransferase OleD variant ASP. Firstly, the glycosyltransferase OleD (ASP) was expressed in Escherichia coli Transetta (DE3) and purified with nickel column. Then curcumin was glycosylated with the purified OleD (ASP), and the biosynthesis products were analyzed by HPLC and LC-MS. It was found that OleD (ASP) could convert curcumin to monoglycoside, diglycoside and triglycoside.

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