Abstract

STUDY OF LPRM GENE IN IRAQI MYCOBACTERIUM TUBERCULOSIS ISOLATES

Asraa A. Abdul-Jalil , Zahra M. Al-Khafaji* , Ahmed A. Mankhi, Ruqaya M. Ali ,Mohammed Q. Al-Ani

ABSTRACT

Tuberculosis (TB) represents one of the most threatening disease in human beings . It caused mainly by Mycobacterium tuberculosis , which gets a lot of virulence factors . The aim of this study was to investigate and study the LprM gene , which accused in internalization of M. tuberculosis in human macrophages . For the Iraqi patients suffering from pulmonary TB tow methods were used to extract the DNA (using CTAB and Triton-X) . The gene amplified with LprM RD7 primers , the resulted PCR product 1116 bp were sequenced for tow typical M. tuberculosis (at phenotypic characterization) (Asraa25R and Asraa28R , were deposited in NCBI with Accession numbers KJ809110 , KJ809111 respectively ) . Resulted sequences and their translated protein sequences were investigated thoroughly using different Bioinformatics tools . Results revealed that 70% of infections in Iraqi patients with pulmonary TB are due to typical M. tuberculosis , which differ from M. tuberculosis complex . The phylogenic estimation put the resulted sequences in mycobacterial group.

Keywords: LprM gene . TB in Iraq , M. tuberculosis , spoligotyping , Rv1970 (RD7) , phylogeny , NCBI Acc. No. KJ809110 , KJ809111