Abstract

STUDY THE BIOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ENTEROTOXIN

Essam F. Al-Jumaily*,Nahla M. Saeed and Hussain H. Khanaka

ABSTRACT

The aim of this study was to investigate the purification of Staphylococcus aureusA20-L2 enterotoxin which induced clinical and sub clinical mastitis in dairy cattle in Sulaimani province. The production of enterotoxin types A, B, C, D, E, G, H, I and J were also tested by PCR technique. Among 246 CoPS isolates, 125 (50.8%) were found to be positive for production of one or more of enterotoxin types A, B, C and E, while none of the CoNS isolates had the ability to produce enterotoxin. Ion exchange-chromatography and gel filtration chromatography were used for the purification of enterotoxin from one isolate of S.aureus which was A20-L2. The molecular weight of the purified enterotoxin was approximately equal to 28.2 kilo Dalton determined by gel filtration chromatography whereas sodium dodecyle sulfate-polyacrylamide gel electrophoresis revealed a single band which was approximately equal to 26 kilo Dalton. Isoelectric focusing was used to determine the isoelectric point (pI) value and it was estimated as 7.

Keywords: Staphylococcus aureus, Enterotoxin, CONS isolates, Purified enterotoxin, molecular weight, Isoelectric point