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Abstract

REVIEW ON ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF LC-MS/MS

Nena S. Jaiswal*, Suvasi S. Patel, Prof. Mitali. Dalwadi, Dr. U. M. Upadhyay

ABSTRACT

Liquid Chromatography/Mass Spectrometry (LC/MS) is fast becoming the preferred tool of liquid chromatographers. It is a powerful analytical technique that combines the resolving power of liquid chromatography with the detection specificity of mass spectrometry. Liquid chromatography (LC) separates the sample components and then introduces them to the mass spectrometer (MS). The MS creates and detects charged ions. The LC/MS data may be used to provide information about the molecular weight, structure, identity and quantity of specific sample components.

Keywords: Liquid Chrmatography/Mass Spectroscopy, Mass spectrometer components, Method Development, Validation parameter.


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