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Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF PHYTOSTEROL FROM ABUTILON INDICUM (L.)SWEET BY HPLC

Sagar S. Sangale*, Dr. M. J. Chavan, Dr. D. S. Chumbhale and Raut Dipak B.

ABSTRACT

Abutilon indicum (L) Medium size branched perennial shrub. Up to 2 meters in height. Plant covered with minute hairs. Leaves are alternate, cordate and acute. Flowers are yellowish with 5 petals. Fruits have 15- 20chambers arranged spirally. Seed colour is blackish brown. Today the development of a method of analysis is usually based on prior art or existing literature, using the same or quite similar instrumentation. It is rare today that an HPLC based method is developed that does not in some way relate or compare to existing, literature based approaches. The development of any new or improved method usually tailors existing approaches and instrumentation to the current analyte, as well as to the final needs or requirements of the method. Abutilon indicum (L) analyse on RP-HPLC to validation parameter like Accuracy, Precision, Specificity, Linearity, Range, Limit of Detection, Limit of Quantitation, Robustness, Ruggedness.

Keywords: .


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