Abstract

A SIMPLE AND VALIDATED RP HPLC METHOD FOR THE ESTIMATION OF ACRYLAMIDE IN POTATO CHIPS

Krishna Veni N*, Meyyanathan S N, Karthik Y, Hemnath E, Satiesh Kumar R,and Usha K

ABSTRACT

A simple rapid High performance liquid chromatographic method was developed for the estimation of acrylamide a potential carcinogen in potato chips. The HPLC method was carried out on a Phenomenex C18 column (250 mm X 4.6 mm id, 5μ) using mobile phase containing Acetonitrile: Water (adjusted to pH 3.5 using orthophosphoric acid) of ratio (20:80 v/v) at a flow rate of 1.0 ml/min. Detection was carried out at 225 nm. Zidovudine was used as an internal standard (IS). The sample preparation was done by solid phase extraction method. The developed method was validated in terms of accuracy, precision, linearity, limit of detection and limit of quantification. The linearity of acrylamide was taken in the range of 1-5 μg/ml and the correlation coefficient was found to be 0.9985. The LOD and LOQ were found to be 20 ng/ml and 40 ng/ml respectively. The content of acrylamide present in eight different varities of potato chips was determined. The proposed method can be used for the estimation of acrylamide in food products containing higher amount of acrylamide.

Keywords: RP-HPLC; Acrylamide; Zidovudine; Solid phase extraction; validation