Abstract

COLLAGEN EXTRACTION OPTIMIZATION FROM THE SKIN OF THE DASYATIS PASTINACA

K. Sundaravalli, K. Revathi*, Adithya R. Pillai and A. Anitha

ABSTRACT

By using the skin of sting ray Dasyatis pastinaca which is thrown as waste by the consumer, processed as a raw material, a macromolecule biomaterial of collagen was extracted. Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were successfully isolated from the skin of string ray Dasyatis pastinaca through extraction methods. The yields of ASC and PSC based on the wet weight of skin were 8.2% and 17.5% (wet weight basis), respectively. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that both the ASC and PSC were Type I collagen and maintained a complete triple helix structure. These results indicated that both ASC and PSC of skin of sting ray Dasyatis pastinaca possessed good biological activity and could be widely used in medical biomaterials and other fields such as cosmetics, tissue engineering, tissue grafting.

Keywords: Dasyatis pastinaca, Acid-soluble collagen (ASC), pepsin-soluble collagen (PSC), medical biomaterials.