Abstract

EMPLOYMENT OF HYDROTROPIC AND MIXED HYDROTROPIC SOLUTIONS AS MOBILE PHASE TO CARRY OUT TLC PRECLUDING THE USE OF ORGANIC SOLVENTS

R. K. Maheshwari, Harshal Wadhwani*, Anuj Jain and Pawan Jat

ABSTRACT

A large number of organic solvents, viz. butanol, acetic acid, hexane, acetone, chloroform, ether, ethyl acetate, ethanol, toluene, dichloromethane, heptane, benzene, methanol, acetonitrile, carbon tetrachloride and cyclohexane are employed to perform thin-layer chromatography (TLC) of various drugs. Most of these organic solvents are costly and hazardous to health. Some organic solvents have been reported to be carcinogenic. To a certain extent, such solvents are responsible for environmental pollution also. Also, their disposal requires stringent procedures which makes the process both costly and typical. In the present investigation, hydrotropic and mixed hydrotropic solutions were employed as mobile phase to perform TLC of some drugs precluding the use of organic solvents. Propranolol hydrochloride, Guaifenesin, Ciprofloxacin hydrochloride, Pyridoxine hydrochloride, Lidocaine hydrochloride, Thiamine hydrochloride, Metformin hydrochloride, Piperazine citrate and Losartan potassium were selected as model drugs. Sodium benzoate, sodium citrate, sodium acetate, & niacinamide in various combinations were employed as model hydrotropic agents. In the case of the proposed methods, solutions of the above listed hydrotropic agents in distilled water in different concentrations were employed as mobile phases to perform TLC of the selected drugs. The observed Rf values in the case of proposed methods ranged from 0.33 to 0.84. As per mixed solvency concept, solids also possess solubilizing properties. In mixed hydrotropy, the composition of blends containing various hydrotropic agents can be varied in such a way that proper Rf value of drug spot is obtained without tailing effect. The proposed TLC methods are new, simple, cost-effective, environment-friendly, and safe. In future, hydrotropic solutions shall prove a boon in TLC and high performance thin layer

Keywords: chromatography (HPTLC) analysis of a vast number of drugs discouraging the use of organic solvents to a great extent.