Abstract

PROBIOTIC ASSESSMENT OF BACTERIA ISOLATED FROM MILK OF DOMESTIC ANIMALS IN PREVENTION OF ENTERIC BACTERIAL INFECTIONS

Aishwarya N. Kapse* and Dr. C. J. Chandekar

ABSTRACT

Probiotics means live microorganisms that have beneficial effects on their host’s health by improving the intestinal microbial balance. A total of 8 milk samples from domestic animals (2 each from Cow, Buffalo, Goat and Sheep) were analyzed Lactobacillus spp. Out of this 8 isolates were identified as prominent probiotics, among them isolates were excellent probiotics. These excellent probiotics were compared for their antibacterial activity of commercial probiotics like Synbio, Sporlac, Rinifol, Vitagut and probiotic bacterial strain Lactobacillus spp. All Lactobacillus spp. were resistant to cephalothin. Lactobacillus spp. were further tested for their antimicrobial activity against common pathogenic bacteria such as Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Salmonella typhi using the agar spot method (Zones of inhibition were measured). Acid tolerance and bacterial antagonistic characteristics of these lactobacillus spp. isolates render them good candidates for consideration as probiotics.

Keywords: Lactobacillus spp., Antibiotic activity, Antagonistic activity, Bacteriocin, Commercial probiotics preparations, Bile salt tolerance, Heat sensitivity.