STUDIES ON THE BIOAVAILABILITY OF ARBORTRISTOSIDE-A IN TABLET FORMULATIONS
*Sanjita Das, D. Sasmal, S.P. Basu
ABSTRACT
Nyctanthus arbortristis Linn. (Family– Oleacea) is a well documented
plant. Its various parts are used traditionally and in folk medicine for
treating various diseases. The ethanolic extract of the leaves, seeds,
bark and flowers (A, B, C and D respectively) were found to have the
LD50 value more than 2.0 gm, whereas of arbortristoside-A was found
to be 750 mg. The extracts and arbortristoside-A are also
hematologically non toxic and have significant anti- inflammatory
activity. The present study is aimed at formulation of the tablet dosage
forms of extracted products (A, B, C, D) and arbortristoside-A and
their bioavailability studies. The tablets were prepared by wet
granulation method and subjected to standard evaluation process. The tablets were tested for
dissolution rate using the USP paddle method. It was observed that the highest rate of release
among the extracts was found with the products A and B (49 .6% and 59.0%) and minimum
release rate was found from the extract of flower (20.1%), whereas that of arbortristoside-A
was found to be 70%. For in-vivo bioavailability studies rats were used and were
administered by the solutions of the tablets. The blood plasma data at different time intervals
gave equivalent comparative results to that of the in-vitro study between the respective
tablets. The system in the GI tract of rats delivered at the same rate as in-vitro which shows
the in vivo test as a bioanalogus method predictive of the in-vitro performance of respective
dosage forms. An in-vivo in-vitro correlation (IVIVC) for tablets of arbortristoside-A was
established. The present study showed that the bioavailability of arbortristoside-A from its
tablets is more than that of the extracts. It may be due to the presence of the chemical
constituents other than arbortristoside-A, interfering its bioavailability.
Keywords: Bioavailability, Nyctanthus arbortristis L, arbortristoside-A.
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