Abstract

IMPORTANCE OF HYPHENATED TECHNIQUES IN BIOANALYSIS

Sarvani P.*

ABSTRACT

Bio-analysis is the quantitative measurement of xenobiotics and biotics (macromolecules, proteins, DNA, large molecule drugs, metabolites) in biological systems. This involves the quantitative determination of drugs and /or metabolites in biological matrices such as blood, serum, plasma or urine, tissue and, skin samples. The analytical methods used for bio-analysis are often referred to as Bio-analytical methods. The growth of new compounds is increasing and leading to a doubling of new chemicals every year. Thus there is a need to efficiently identify substances and quantitate material with a high degree of accuracy. Hyphenated instruments are the combinations of accepted instrumental techniques where the sample is passed from one instrument directly into another using a proper interface. These techniques were developed to aid in solving the problem. Hyphenated analytical methods are the most commonly used bio-analytical methods. These techniques provide more complementary information in a shorter time leading to faster and more reliable results than data obtained from traditional instrumental methods. The main purpose of bio-analysis in the pharmaceutical industry is to obtain a quantitative measure of the drug and its metabolites in bioequivalence, pharmacokinetic, and toxicokinetic studies to study the exposure data. The present article reviews about different hyphenated techniques used for bio-analysis with their importance in recent trends.

Keywords: Bioanalysis, Hyphenated techniques, Chromatrography, Spectrometry, LC-MS, GC-MS, LC-NMR, LC-IR, CE-MS.