Abstract

IN-VITRO PHYTOPHARMACOLOGICAL AND ANTICANCER ACTIVITY OF WEDELIA CHINESIS AGAINST HUMAN BREAST CANCER CELLS (MCF-7)

Nithin Manohar R.*, Padmaja V., R. Rajkumar, Shiji Kumar P. S. and Ancy P.

ABSTRACT

In this study, we evaluated anticancer activity of ethanol extract of Wedelia chinesis (W. chinesis) against Human breast cancer cells (MCF-7). Cytotoxicity of W.chinesis was investigated using MTT assay. Doxorubicin was used as the positive control. MTT assay showed activity against the tested cell line. The cytotoxicity activities were expressed as percentage of cell viability. Cytotoxicity of ethanolic extract of W.chinesis against MCF-7 cell line was measured and the IC50 value of ethanol extract of W.chinesis was 81.74 ±0.211 μg/mL μg/ml. IC50 value of Doxorubicin (standard) was 13.53±0.18 μg/mL. Morphological alteration of MCF-7 cells lines upon exposure using W.chinesis extract was observed under phase contrast microscope. The cells indicated the most prominent effects after exposure to the W.chinesis extract.The present findings confirmed cytotoxic effect of ethanol extract of W.chinesis Human breast cancer cells (MCF-7).

Keywords: Medicinal plants, herbs, anticancer, cell lines, cytotoxicity, flavanoids, polyphenolic compounds.