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Nikita Tiwari*, Dr. M. H. Ghante, Prof. Dr. N. B. Ghiware, Dr. S. K. Sarje


The present study reports physicochemical characterization, antioxidant and Memory enhancing activity of extracts from Cyamopsis tetragonoloba L. pods collected from local region of Nanded, Maharashtra, India. Different physical parameters like ash values, extractive value, Loss on drying, solubility etc were evaluated for powdered drug. The extracts were obtained from Soxhlet method by using water and methanol as solvents for extraction and subjected for preliminary physicochemical evaluation and antioxidant studies. Total phenolic and flavonoids content were also analyzed. The presence of primary and secondary metabolites such as carbohydrate, proteins, alkaloids, phenolic compounds, saponins was confirmed through preliminary phyto-chemical analysis. DPPH free radical scavenging assays showed strong antioxidant activities with increase in concentration of Ethyl acetate and methanol leaves extracts. Maximum percentage inhibition i.e. 76.14 % was shown by Methanolic extract at concentration of 150 μg/ml and was compared with Ascorbic acid as reference standard.The In-Vivo memory enhancing activity of Cyamopsis tetragonoloba L. pods was evaluated by radial arm maze model in rats using Piracetam as a standard. Both the extracts at 200mg/kg concn showed significant to highly significant number of entries & time spent in P zone (from P < 0.05 to P < 0.001).The result suggests that Cyamopsis tetragonoloba L. pods extracts possess memory enhancing activity and this might be due to flavonids. Phenolic compound, steroid and proteins present in extract.

Keywords: Cyamopsis tetragonoloba L. pods, Ethyl acetate and Methanolic extract, Phytochemical screening, Antioxidant effect, Memory enhancing activity.

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