Abstract

CHARACTERIZATION AND DEVELOPMENT OF EMBELIA RIBES PHYTOSOMES

Chanchal Jain*, Dr. A. K. Singhai and Sonal Gupta

ABSTRACT

Embelia ribes, in particular embelin isolated from dried berries of embelia ribes has a wide spectrum of biological activities. Belong to the family Myrsinacae It is commonly known as false black pepper or Vidanga it is widely distributed throughout india.in ayurveda and siddha it is considered widely beneficial in variety of disease. E. Ribes Seeds are rich in alkaloids, flavonoids and phenolic compounds Hence,in present work we have analyzed The DPPH free radical scavenging activity of Hydroalcoholic extract of Embelia ribes were 21.64%, 27.43%, 32.39%, 37.36%, 41.25%, 49.96% at different concentration of 10 μg/ml, 20 μg/ml, 40 μg/ml, 60 μg/ml, 80 μg/ml, 100 μg/ml respectively. The Embelia ribes seed extract has been showed the robust scavenging activity at 100μg/ml, which was 49.96% as compared to Ascorbic acid 84.13%. This activity was lower than ascorbic acid, Embelia ribes hydroalcoholic extract exhibited potent scavenging activity (IC50-103.93). Reference standard ascorbic acid showed IC50 -17.681. In this study, we prepared the, Embelia ribes -phospholipids complex to improve the lipophilic properties of Embelia ribes. We prepared the complex with different quantity ratios of phospholipids: cholesterol and Embelia ribes such as 1:1:1, 1:2:1, 2:1:1, 2:3:1.The phytosomes formulation having 2:1 ration of phospholipid and cholesterol and 1.0% w/v extract of embelia ribes (F10) showing the greatest entrapment efficiency,(73.32+0.25) with small particle size (313.25) was further evaluated for drug release study. The phytosomal formulation of Embelia ribes (a complex of E.ribes with phosphatidylcholine) has been shown to improve E.ribes bioavailability.

Keywords: embelia ribes, phytosomes, antioxidant activity antidiabetic, hydro alcoholic extraction.