Abstract

VALIDATION OF ANALYTICAL METHODS AND DETERMINATION OF ALPHA-LINOLENIC ACID (OMEGA 3) AND LINOLEIC ACID (OMEGA 6) IN SOME FORMULA MILK PRODUCT

Azfrianty, Regina Andayani and Harrizul Rivai*

ABSTRACT

In certain conditions, formula milk, if necessary, can be used as a substitute for breast milk, which must have formulations comparable to breast milk. Alpha-linolenic acid (ALA) and linoleic acid (LA) are essential fatty acids that must be contained in formula milk based on The Regulation of Indonesian FDA, Number 1 of 2018. This study aims to obtain valid, simple, and reproducible ALA and LA analysis methods to guarantee the quality and safety of formula milk. Validation of ALA and LA analysis methods using AOCS Official Methods Ce 2b-11 modified in formula milk by gas chromatography using the capillary column DB Fast FAME. The analysis method meets the validation requirements. ALA had quantitation limit detection as low as 6.833 μg/mL, repeatability (RSD) less than 1.9 %, and recovery from 96.2 % to 103.9 %. LA had quantitation limit detection as low as 8.063 μg/mL, repeatability (RSD) less than 0.9 %, and recovery from 97.4 % to 99.2 %. This method was applied to determine the content of ALA and LA in 11 sample formula milk products. The analyzed contents of ALA between 50.850 ± 0.053 mg/100 kcal to 122.279 ± 0.113 mg/100 kcal and LA between 478.152 ± 0.267 mg/100 kcal to 956.606 ± 0.207 mg/100 kcal. This result showed that the samples contained ALA and LA by applicable requirements. However, by evaluating the label declaration, three samples contain ALA and four samples containing LA lower than label declaration.

Keywords: Capillary column DB Fast FAME, alpha-linolenic acid, linoleic acid, bomb calorimeter, and formula milk.