Abstract

A STUDY ON THE EXTRACTION, ISOLATION AND CHARACTERIZATION OF FLAVONOIDS FROM COCCULUS HIRSUTUS

Sheela S. M.* and Seema Gupta

ABSTRACT

The plant Chilihindah, Cocculus hirsutus Linn. Belongs to family Menispermaceae. In Ayurvedic system of medicine the use of this plant has been described in diverse diseases. The roots and leaves are reported to possess antimicrobial, laxative, demulcent, diuretic, cardiotonic and antidiabetic activity. The phytochemical investigation included successive extraction with pet ether 40-60oC, chloroform, alcohol and maceration by water and alcohol. The extracts were subjected to preliminary phytochemical screening, chromatographic studies and isolation of phytoconstituents. Preliminary phytochemical screening of various extracts showed the presence of alkaloids, flavonoids, carbohydrates, sterols, glycosides, gum and mucilage, fixed oil and fats. TLC study for the different phyto-constituent and HPTLC for alcoholic extract was performed. The Ethyl acetate fraction of methanolic extract showed the maximum free radical scavenging capacity as compared to other fractions. Two bioactive flavonoids were isolated from the Ethyl acetate fraction by using column chromatography. The isolated flavonoids were subjected to spectral analysis 1H-NMR and FT-IR.

Keywords: Cocculus hirsutus, Flavonoids, column chromatography, HPTLC.