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S.S. Tikole*, T.B.Kakade, D.B.Shelar, G.S. Bamane, M.V. Bite, P.A.Shelar


Development is being made on numerous fronts to introduce new traits into plants using recombinant DNA technology. Genetic engineering promises to speed the process and broaden the scope. Several different genetic modifications have been used to engineer tolerance to insects, fungus and bacterial and herbicides, the most widely adopted genetic engineering trait overall. Transgenic plant research depends on the availability of procedures for plant transformation. Two types of method for plant transformation exist, the use of Agrobacterium as a biological vector for foreign gene transfer, and direct gene transfer techniques, in which DNA is introduced into cells by the use of physical, electrical or chemical means. New transgenic varieties have been produced that are resistant to pathogens, insects, herbicides, or express novel characters that improve product quality and agronomic traits. These technologies should be used to complement the traditional methods for enhancing productivity and quality rather than to replace the conventional breeding methods. Now a day, ripening of fruits, prevention of depolarization, increasing size of fruits, to make disease free plant against insect, bacteria, fungus and virus etc are new successful approaches to our nation like Bt cotton, Bt Maize, Flavr Savr Tomato, Golden Rice etc.

Keywords: Agrobacterium, recombinant DNA technology, Genetic engineering.

[Full Text Article]

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