Abstract

COMPARISON OF THE ANTIOXIDANT ACTIVITY OF THREE HYDROETHANOLIC EXTRACTS FROM BLIGHIA SAPIDA (SAPINDACEAE)

Honoré Yao N’guessan*, Cassime Tiemoko, Timothé Aboua Okpékon, Fatogoma Sorho, Alain Dit Philippe Bidié and Daouda Koné

ABSTRACT

The aim of this work was to compare the in vitro antioxidant activity of three hydroethanolic extracts of Blighia sapida a sapindaceae from the Ivorian pharmacopoeia. These extracts were obtained by maceration in a 70% hydroethanolic solution for 48 hours at room temperature. After successive filtrations on hydrophilic cotton and Wattman paper, followed by evaporation using a rotary evaporator, the hydroethanolic extracts collected were used to carry out the triphytochemical study and the antioxidant activity. The triphytochemical was carried out using colorimetric methods and precipitation tests, then the antioxidant activity by DPPH, by the FRAP method and by the ABTS radical-cation were used. The phytochemical screening revealed the sterols, polyterpenes, polyphenols, flavonoids, quinone substances and gallic tannins contained in Blighia sapida. The results showed that the hydroethanolic extract of Adzopé has the highest concentrations of total polyphenols and flavonoids compared to the extracts of Bouaké and Korhogo. Also, the hydroethanolic extract of Adzopé showed a higher antioxidant and antiradical activity than the hydroethanolic extracts of Bouaké and Korhogo. These activities of Adzopé extract are attributed to its high content of polyphenols and total flavonoids which are generally very good antioxidants. These antioxidant and antiradical activities of hydroethanolic extracts differ from one area to another and also from one climate to another. The hydroethanolic extracts of Blighia sapida could therefore be used in the cosmetic, food and pharmaceutical industries for the formulation of various products with a therapeutic purpose.

Keywords: Blighia sapida, antioxidant, FRAP, ATBS, DPPH, antiradical.