Abstract

EFFECT OF MICROENCAPSULATION TECHNIQUES WITH HPMC POLYMERS ON THE ACTIVITY AND STABILITY OF CRUDE BROMELAIN FROM PINEAPPLE STEM (ANANAS COMOSUS (L.) MERR)

Rose Intan Perma Sari*, Erizal Zaini and Salman Umar

ABSTRACT

The objective of this research was to determine the effect on the stability of crude bromelain coating mikroencapsulation results by emulsification solvent evaporation method. The type of coating used was HPMC made in 3 formulas with a ratio of the coating and the active substance of 0.5: 1, 1: 1, 1.5: 1. The microcapsules obtained are evaluated include the evaluation of FT-IR spectrophotometers, particle size distribution, the entrapment efficiency (EE), water content, SEM, dissolution profile, the protein concentration, enzyme activity and followed by stability testing at room temperature for 3 months. The analysis result spectrophotometer FT-IR showed no interaction between the active substance with the polymer, analysis SEM shows all the microcapsules in each formula is round (spherical), the efficiency of adsorption depends on the concentration of the HPMC, results of dissolution showed a slowdown in the release of crude bromelain in microcapsules the whole formula obtained has met the requirements for particle size and particle size distribution of the microcapsules is almost evenly and stability tests showed reduced levels of 93.99% -89.86%. This study revealed that the microcapsule formulation with HPMC and crude bromelain can maintain enzyme activity and stability of bromelain rugged and can decrease the dissolution rate.

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