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Abstract

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF PRUCALOPRIDE SUCCINATE IN PHARMACEUTICAL DOSAGE FORM

Vaibhavi N. Akhani*, Mrs. Khushbu K. Patel, Ms. K. S. Patel, Dr. L.M. Prajapati and Dr. C. N. Patel

ABSTRACT

A Simple, Precise, and Accurate RP-HPLC method was developed and validated for the estimation of Prucalopride Succinate in tablet dosage form. The method was achieved on waters C18 (150 × 4.6 mm, 5μ). The method showed a linear response in the concentration range of 10-50 μg/mL using methanol: water as mobile phase in the ratio of 70: 30 v/v with detection at 226 nm with flowrate of 1 mL/min and retention time was 2.9 min. the method was validated as per ICH guideline. The method was successfully applied for routine analysis of Prucalopride Succinate in tablet dosage form.

Keywords: Prucalopride Succinate, RP-HPLC, validation, Pharmaceutical dosage form.


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