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Abstract

SIMULTANEOUS HPTLC DETERMINATION OF CAMYLOFIN DIHYDROCHLORIDE AND NIMESULIDE IN PHARMACEUTICAL FORMULATION

*RajeevKumar R. Singh, Manatragada V. Rathnam

Department of Chemistry, B.N. Bandodkar College of Science, Chendani, Thane- 400601, Maharashtra, India. 

ABSTRACT

An HPTLC method for simultaneous determination of Camylofin Dihydrochloride and Nimesulide in a pharmaceutical formulation has been developed and validated. The analytes were separated on silica gel 60F254 HPTLC plates with Benzene: Methanol: Ammonia in the ratio of 7.5:2.5:0.1, as mobile phase, after chamber saturation for 15 min. The development distance was 9 cm. The plate was then dried in air and scanned and quantified at the wavelength at 220 nm. The limit of detection were of Camylofin dihydrochloride and Nimesulide was found to be 38 μg mL-1 & 15 μg mL-1 respectively. The limit of quantification of Camylofin dihydrochloride and Nimesulide was found to be 72 μg mL-1 & 39 μg mL-1 respectively. The method enables accurate, precise, and rapid simultaneous analysis of Camylofin Dihydrochloride and Nimesulide. It can be conveniently adopted for routine quality control analysis.

Keywords: HPTLC, Camylofin Dihydrochloride, Nimesulide.


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