Abstract

ETHYL-4-(DIMETHYLAMINO)-2-(4-METHOXYPHENYL) PROPANOTE AS ANXIOLYTIC, ANTI-INFLAMMATORY AND ANTI-BACTERIAL AGENT

Hasti Kenia*, B. Shivkumar, Ramaling B. Kotnal, Ramesha A. Ramakrishnan, Pradeep Devadiga, Chandrashekhar C. Simpi and Chandrshekar V. M.

ABSTRACT

An improved process for the synthesis of Ethyl-4-(dimethylamino)-2-(4-methoxyphenyl)propanote was carried out by hydrolysis of 2-(4-methoxyphenyl)acetonitrile using sodium hydroxide to obtain 2-(4-methoxyphenyl)acetic acid. 2-(4-methoxyphenyl)acetic acid was then esterified with ethanol to obtain 2-(4-methoxyphenyl)acetate. 2-(4-methoxyphenyl)acetate then undergoes Mannich reaction with paraformaldehyde and dimethylamine in presence of catalytic amount of tertiary butyl ammonium bromide. The synthesized compounds were characterized by IR, 1H NMR and 13C NMR. The final compound was then subjected to purity analysis by HPLC. Also, the final compound was then subjected to pharmacological screening for anxiolytic activity by Elevated plus maze test, Open field test and Motor co-ordination test by Rota rod. The compound synthesized was also screened for in-vitro anti-inflammatory activity by protein denaturation method and antibacterial activity by serial dilution method.

Keywords: Mannich reaction, Anxiolytic, Anti-inflammatory, Anti-bacterial, Serial dilution method.