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Abstract

HPTLC FINGERPRINTING ANALYSIS OF TRITERPENOIDS IN BAUHINIA PURPUREA L. FROM DIFFERENT GEOGRAPHICAL REGIONS OF INDIA

Aparna Saraf* and Smita Narayan

ABSTRACT

The study was designed to establish the HPTLC fingerprints of triterpenoids for leaf and bark of the medicinally important plant Bauhinia purpurea L. collected from three different geographical regions of India viz., Mumbai, Mangaluru and Bengaluru (Karnataka). Preliminary phytochemical screening was done followed by the HPTLC studies. n-Hexane: Ethyl Acetate (1:1) (v/v) was employed as the mobile phase for the separation of triterpenoids. The derivatization reagent used was Anisaldehyde Sulphuric Acid reagent was suitable to generate more bands of triterpenoids. A better separation of triterpenoids was observed at 540 nm after derivatization. The study established diverse varieties of triterpenoids in large numbers in the bark and leaf of Bauhinia purpurea L. from all the three regions along with some variations in them. It can be concluded that HPTLC fingerprint analysis of bark and leaf extract of Bauhinia purpurea L.can be used as a diagnostic tool for the accurate identification of the plant and also it is useful as a phytochemical marker.

Keywords: Bauhinia purpurea L., triterpenoids, HPTLC fingerprint.


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