Abstract

PREPARATION AND EVALUATION OF LIPOSOMAL GEL OF MUPIROCIN CALCIUM FOR DEEPER SKIN INFECTION

*Qureshi Subhana, Khan Mohd. Faarooq and Bhardwaj Rita

ABSTRACT

Mupirocin calcium is a unique antimicrobial agent because of it's structure and mechanism of action. The drug exerts its antimicrobial activity by reversibly inhibiting isoleucyl-transfer RNA, thereby inhibiting bacterial protein and RNA synthesis. this research designed to develop efficient topical therapeutic activity in pharmaceutical system to enhance its transdermal penetration in comparison to simple gel of drug. this formulation is prepared in different batches ELSL-1 to ELSL-7 by using carbopol 934, propylene glycol, NAOH etc. drug identification and preformulation study have been done by melting point, FTIR, partition coefficient, calibration curve etc. the formulation characterized by vesicle size, surface charge and vesicle size, entrapment efficiency, pH measurement, viscosity measurement, drug content, extrudability study, spreadiblity, in vitro drug diffusion study, stability study. it is thus concluded that formulation (ELSL-6) was said to be optimized formulation, it were found the viscosity of gel was 178.37±5.07 CPS, entrapment efficiency was 71.03±2.49%, extrudability was 170g and spreading found that 5.16(g.cm/sec) respectively. in vitro drug release from elastic liposomes was carried out using Firenze diffusion cell method and found 41.36±1.8 in 24 hrs, 52.06±2.6 in 48 hrs and 61.6±3.2% in 72 hrs, the UV spectroscopy of mupirocin calcium exhibited absorbance Maxima (λ max) at 227nm in 7.4 pH buffer. the results obtained may be considered as tools for the manufacturer of standard formulation with great efficacy.

Keywords: Mupirocin calcium, gel, formulation, ELSL.