Abstract

EFFECT OF GLIMEPIRIDE ON REDUCING BLOOD SUGAR LEVELS IN MALE WHITE MICE WITH DIABETES MELLITUS WITH COMPLICATIONS OF LIVER DYSFUNCTION

Lili Sartika, Helmi Arifin, Almahdy A. and Harrizul Rivai*

ABSTRACT

This research has been carried out to determine the effect of glimepiride on reducing blood sugar in diabetic white male mice with complications of liver dysfunction. Diabetic mice are made by injecting alloxan. Diabetic mice with complications of liver dysfunction are made by injecting alloxan and carbon tetrachloride. Mice were divided into seven groups, namely positive control groups and six groups of test preparations given glimepiride for 15 days (1, 2, and 4 mg/kg BW). The results showed that administration of glimepiride 1 mg, 2 mg and 4 mg in the diabetic and diabetic-liver dysfunction group decreased blood sugar levels were 58.3 mg/dL and 41.2 mg/dL; 93.5 mg/dL and 67.1 mg/dL; 118.93 mg/dL and 89.6mg/dL. Decrease in blood sugar levels on day 5, day 10 and day 15 of the diabetes group dose of 1 mg, 2 mg and 4 mg were 61 mg/dL, 86.20 mg/dL and 133.20 mg/dL, the diabetic-liver dysfunction group respectively were 69.8 mg/dL, 133.2 mg/dL and 153.80 mg/dL. In diabetic-liver dysfunction mice the decrease in glucose levels in glimepiride blood 1 mg respectively was 27 mg/dL, 44 mg/dL and 52.6 mg/dL, 2 mg respectively was 44 mg/dL, 55 mg/dL and 102.2 mg/dL, 4 mg respectively were 57.8 mg/dL, 102.2 mg/dL and 108.8 mg/dL. From the results of this study, it can be concluded that the mechanism of action of glimepiride is affected by the length of treatment and is not influenced by the pathological disease.

Keywords: Diabetes mellitus, liver dysfunction, glimepiride, alloxan, carbon tetrachloride, blood sugar.