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Abstract

ANTIBACTERIAL ACTIVITY OF AERIAL PARTS EXTRACT OF JASMINUM OFFICINALE & KLEINIA BALSAMICA

Vijay Nigam*, Prashant Gupta, Shruti Mittal and Sanjay Kumar Jain

ABSTRACT

The antibacterial activity of crude petroleum ether, benzene, chloroform, acetone, ethanol and aqueous extracts of Jasminum officinale (Family: Oleaceae) & Kleinia balsamica (Family: Compositae). Aerial parts were tested against Staphylococcus capitis, Staphylococcus mutans, Pseudomonas mirabilis and Bacillus fragillis. The in vitro antibacterial activity was performed by agar disc diffusion method. The zone of inhibition was compared with the standard drug i.e. Penicillin. Petroleum ether, chloroform, acetone and ethanol extracts were effective against the entire four test microorganism used respectively when compared to standard drug penicillin. The minimum inhibitory concentration [MIC] for S.capitis was 10,750,10,10,10 and 1000 mg/ml; MIC for S. mutans was 10,125,10,10,10 and 10 mg/ml; MIC for P.mirabilis was 20, 10,10,10,20 and 10 mg/ml and MIC for B. fragillis was 10,10,10,20,10 and 20 mg/ml for petroleum ether, benzene, chloroform, acetone, ethanol and aqueous extracts respectively suggesting the antibacterial activity of Jasminum officinale & Kleinia balsamica. Acetone extract was more effective followed by ethanol extract as antibacterial agents when compared to other extracts of aerial parts of Jasminum officinale & Kleinia balsamica. Work is under progress to reveal the chemical nature of the active constituents responsible for the antibacterial activity.

Keywords: Aerial parts, antibacterial activity, Jasminum officinale & Kleinia balsamica, minimum inhibitory concentration, zone of inhibition.


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